Agenda For PU1

TODO Create UMAP and PCA (Part B) [2/3]

  • Molly made the samplesheets manually so need to regenerate with code to add more metadata columns
  • Matt wants supplemental figures for antibody and experiment
  • Antibody already has label, but need experiment annotations
  • [X] Antibody supplement
  • [X] Set colors to match cell type diagram

Figure 2

TODO GREAT analysis (Part D) [1/4]

  • In the Figures folder
  • Where did bed files come from?
  • [X] Emailed Lee about code
  • Use fold enrichment instead of odds ratio
  • Generate Tables
  • Integrate great script with makefile?

Figure 3

TODO Part A-B

  • Move SPI1/ets to bottom of bar plot

TODO SPAMO analysis (Part C)

  • Identifies likely partners for PU.1
  • May or may not be working on it?

Figure 4

TODO Phenotype RELI (Part A)

  • Needs new GWAS catalogue
  • Leah Released GWAS catalogue (how do I get it?)

Shreeja is the GWAS gatekeeper.

– Matt

TODO Mario Analysis

  • Identifies allele dependant behavior

TODO Download EGA datasets (vcf files)

  • Required for MARIO analysis
  • Need Permission still (shreeja will know)

DONE Draft Methods

-Note taken on [2025-06-03 Tue 13:06]
Added to wiki at https://tfwiki.cchmc.org/wiki/PU.1_Project/Methods

  • Put in Docs folder
  • Maybe put on wiki instead?

Papers to Read

TODO Harley et al

Want to, but don't need to?

TODO Dockerize/Nixify R dependencies

  • Are we publishing code?