• post-doc under Matt

VTR project (Matt Hass)

• making stable HEK cells

Initial steps

• Alphafold
• transient transfection

VZV ORF29

VZV IE63

• very little structure

Molly

• MPRA atopic dermatitis
• Paper in review
• EMSA with recombinant JuneB (AP1) protein?

EMSA with recombinant JuneB proteins

• dissapointing (didn't bind)
• should like to bind AP1 proteins and homerdimerizes
• Motif is found, but not through EMSA
• No positive control
• Is recombinant protein functional?
• AP1-ChIP

Lois

• MPRA (genotype dependant binding)
• CRISPR interfenerence screen
• Wittle down plates
• Knockdown efficiency of different gRNAs?
• eQTL scores
• Jerkit Cells?
• Nextflow pipeline

Areme Diouf

• vTR HSV1
• vTR CMV
• vTR KSHV

Western Blots

• Messy but informative